Tuning Catalytic Bias of Hydrogen Gas Producing Hydrogenases.
Identifieur interne : 000007 ( Main/Exploration ); précédent : 000006; suivant : 000008Tuning Catalytic Bias of Hydrogen Gas Producing Hydrogenases.
Auteurs : Jacob H. Artz [États-Unis] ; Oleg A. Zadvornyy [États-Unis] ; David W. Mulder [États-Unis] ; Stephen M. Keable [États-Unis] ; Aina E. Cohen [États-Unis] ; Michael W. Ratzloff [États-Unis] ; S Garrett Williams [États-Unis] ; Bojana Ginovska [États-Unis] ; Neeraj Kumar [États-Unis] ; Jinhu Song [États-Unis] ; Scott E. Mcphillips [États-Unis] ; Catherine M. Davidson [États-Unis] ; Artem Y. Lyubimov [États-Unis] ; Natasha Pence [États-Unis] ; Gerrit J. Schut [États-Unis] ; Anne K. Jones [États-Unis] ; S Michael Soltis [États-Unis] ; Michael W W. Adams [États-Unis] ; Simone Raugei [États-Unis] ; Paul W. King [États-Unis] ; John W. Peters [États-Unis]Source :
- Journal of the American Chemical Society [ 1520-5126 ] ; 2020.
Abstract
Hydrogenases display a wide range of catalytic rates and biases in reversible hydrogen gas oxidation catalysis. The interactions of the iron-sulfur-containing catalytic site with the local protein environment are thought to contribute to differences in catalytic reactivity, but this has not been demonstrated. The microbe Clostridium pasteurianum produces three [FeFe]-hydrogenases that differ in "catalytic bias" by exerting a disproportionate rate acceleration in one direction or the other that spans a remarkable 6 orders of magnitude. The combination of high-resolution structural work, biochemical analyses, and computational modeling indicates that protein secondary interactions directly influence the relative stabilization/destabilization of different oxidation states of the active site metal cluster. This selective stabilization or destabilization of oxidation states can preferentially promote hydrogen oxidation or proton reduction and represents a simple yet elegant model by which a protein catalytic site can confer catalytic bias.
DOI: 10.1021/jacs.9b08756
PubMed: 31816235
Affiliations:
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Artz</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 </wicri:regionArea><wicri:noRegion>Washington 99164 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 </wicri:regionArea><wicri:noRegion>Colorado 80401 </wicri:noRegion></affiliation></author><author><name sortKey="Zadvornyy, Oleg A" sort="Zadvornyy, Oleg A" uniqKey="Zadvornyy O" first="Oleg A" last="Zadvornyy">Oleg A. Zadvornyy</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 </wicri:regionArea><wicri:noRegion>Washington 99164 </wicri:noRegion></affiliation></author><author><name sortKey="Mulder, David W" sort="Mulder, David W" uniqKey="Mulder D" first="David W" last="Mulder">David W. Mulder</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 </wicri:regionArea><wicri:noRegion>Colorado 80401 </wicri:noRegion></affiliation></author><author><name sortKey="Keable, Stephen M" sort="Keable, Stephen M" uniqKey="Keable S" first="Stephen M" last="Keable">Stephen M. Keable</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 </wicri:regionArea><wicri:noRegion>Montana 59717 </wicri:noRegion></affiliation></author><author><name sortKey="Cohen, Aina E" sort="Cohen, Aina E" uniqKey="Cohen A" first="Aina E" last="Cohen">Aina E. Cohen</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Ratzloff, Michael W" sort="Ratzloff, Michael W" uniqKey="Ratzloff M" first="Michael W" last="Ratzloff">Michael W. Ratzloff</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 </wicri:regionArea><wicri:noRegion>Colorado 80401 </wicri:noRegion></affiliation></author><author><name sortKey="Williams, S Garrett" sort="Williams, S Garrett" uniqKey="Williams S" first="S Garrett" last="Williams">S Garrett Williams</name><affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 </wicri:regionArea><wicri:noRegion>Arizona 85287 </wicri:noRegion></affiliation></author><author><name sortKey="Ginovska, Bojana" sort="Ginovska, Bojana" uniqKey="Ginovska B" first="Bojana" last="Ginovska">Bojana Ginovska</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Northwest National Laboratory , Richland , Washington 99352 </wicri:regionArea><wicri:noRegion>Washington 99352 </wicri:noRegion></affiliation></author><author><name sortKey="Kumar, Neeraj" sort="Kumar, Neeraj" uniqKey="Kumar N" first="Neeraj" last="Kumar">Neeraj Kumar</name><affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Northwest National Laboratory , Richland , Washington 99352 </wicri:regionArea><wicri:noRegion>Washington 99352 </wicri:noRegion></affiliation></author><author><name sortKey="Song, Jinhu" sort="Song, Jinhu" uniqKey="Song J" first="Jinhu" last="Song">Jinhu Song</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Mcphillips, Scott E" sort="Mcphillips, Scott E" uniqKey="Mcphillips S" first="Scott E" last="Mcphillips">Scott E. Mcphillips</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Davidson, Catherine M" sort="Davidson, Catherine M" uniqKey="Davidson C" first="Catherine M" last="Davidson">Catherine M. Davidson</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Lyubimov, Artem Y" sort="Lyubimov, Artem Y" uniqKey="Lyubimov A" first="Artem Y" last="Lyubimov">Artem Y. Lyubimov</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Pence, Natasha" sort="Pence, Natasha" uniqKey="Pence N" first="Natasha" last="Pence">Natasha Pence</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 </wicri:regionArea><wicri:noRegion>Washington 99164 </wicri:noRegion></affiliation></author><author><name sortKey="Schut, Gerrit J" sort="Schut, Gerrit J" uniqKey="Schut G" first="Gerrit J" last="Schut">Gerrit J. Schut</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 </wicri:regionArea><wicri:noRegion>Georgia 30602 </wicri:noRegion></affiliation></author><author><name sortKey="Jones, Anne K" sort="Jones, Anne K" uniqKey="Jones A" first="Anne K" last="Jones">Anne K. Jones</name><affiliation wicri:level="1"><nlm:affiliation>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 </wicri:regionArea><wicri:noRegion>Arizona 85287 </wicri:noRegion></affiliation></author><author><name sortKey="Soltis, S Michael" sort="Soltis, S Michael" uniqKey="Soltis S" first="S Michael" last="Soltis">S Michael Soltis</name><affiliation wicri:level="1"><nlm:affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 </wicri:regionArea><wicri:noRegion>California 94025 </wicri:noRegion></affiliation></author><author><name sortKey="Adams, Michael W W" sort="Adams, Michael W W" uniqKey="Adams M" first="Michael W W" last="Adams">Michael W W. Adams</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 </wicri:regionArea><wicri:noRegion>Georgia 30602 </wicri:noRegion></affiliation></author><author><name sortKey="Raugei, Simone" sort="Raugei, Simone" uniqKey="Raugei S" first="Simone" last="Raugei">Simone Raugei</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 </wicri:regionArea><wicri:noRegion>Washington 99164 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Northwest National Laboratory , Richland , Washington 99352 </wicri:regionArea><wicri:noRegion>Washington 99352 </wicri:noRegion></affiliation></author><author><name sortKey="King, Paul W" sort="King, Paul W" uniqKey="King P" first="Paul W" last="King">Paul W. King</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 </wicri:regionArea><wicri:noRegion>Colorado 80401 </wicri:noRegion></affiliation></author><author><name sortKey="Peters, John W" sort="Peters, John W" uniqKey="Peters J" first="John W" last="Peters">John W. Peters</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 </wicri:regionArea><wicri:noRegion>Washington 99164 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 </wicri:regionArea><wicri:noRegion>Montana 59717 </wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Northwest National Laboratory , Richland , Washington 99352 </wicri:regionArea><wicri:noRegion>Washington 99352 </wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of the American Chemical Society</title><idno type="eISSN">1520-5126</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Hydrogenases display a wide range of catalytic rates and biases in reversible hydrogen gas oxidation catalysis. The interactions of the iron-sulfur-containing catalytic site with the local protein environment are thought to contribute to differences in catalytic reactivity, but this has not been demonstrated. The microbe <i>Clostridium pasteurianum</i> produces three [FeFe]-hydrogenases that differ in "catalytic bias" by exerting a disproportionate rate acceleration in one direction or the other that spans a remarkable 6 orders of magnitude. The combination of high-resolution structural work, biochemical analyses, and computational modeling indicates that protein secondary interactions directly influence the relative stabilization/destabilization of different oxidation states of the active site metal cluster. This selective stabilization or destabilization of oxidation states can preferentially promote hydrogen oxidation or proton reduction and represents a simple yet elegant model by which a protein catalytic site can confer catalytic bias.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">31816235</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-5126</ISSN><JournalIssue CitedMedium="Internet"><Volume>142</Volume><Issue>3</Issue><PubDate><Year>2020</Year><Month>01</Month><Day>22</Day></PubDate></JournalIssue><Title>Journal of the American Chemical Society</Title><ISOAbbreviation>J Am Chem Soc</ISOAbbreviation></Journal><ArticleTitle>Tuning Catalytic Bias of Hydrogen Gas Producing Hydrogenases.</ArticleTitle><Pagination><MedlinePgn>1227-1235</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/jacs.9b08756</ELocationID><Abstract><AbstractText>Hydrogenases display a wide range of catalytic rates and biases in reversible hydrogen gas oxidation catalysis. The interactions of the iron-sulfur-containing catalytic site with the local protein environment are thought to contribute to differences in catalytic reactivity, but this has not been demonstrated. The microbe <i>Clostridium pasteurianum</i> produces three [FeFe]-hydrogenases that differ in "catalytic bias" by exerting a disproportionate rate acceleration in one direction or the other that spans a remarkable 6 orders of magnitude. The combination of high-resolution structural work, biochemical analyses, and computational modeling indicates that protein secondary interactions directly influence the relative stabilization/destabilization of different oxidation states of the active site metal cluster. This selective stabilization or destabilization of oxidation states can preferentially promote hydrogen oxidation or proton reduction and represents a simple yet elegant model by which a protein catalytic site can confer catalytic bias.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Artz</LastName><ForeName>Jacob H</ForeName><Initials>JH</Initials><Identifier Source="ORCID">0000-0001-7661-2538</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zadvornyy</LastName><ForeName>Oleg A</ForeName><Initials>OA</Initials><Identifier Source="ORCID">0000-0002-5374-6856</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mulder</LastName><ForeName>David W</ForeName><Initials>DW</Initials><AffiliationInfo><Affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Keable</LastName><ForeName>Stephen M</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cohen</LastName><ForeName>Aina E</ForeName><Initials>AE</Initials><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ratzloff</LastName><ForeName>Michael W</ForeName><Initials>MW</Initials><AffiliationInfo><Affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Williams</LastName><ForeName>S Garrett</ForeName><Initials>SG</Initials><Identifier Source="ORCID">0000-0003-4027-4770</Identifier><AffiliationInfo><Affiliation>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ginovska</LastName><ForeName>Bojana</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0003-0165-8202</Identifier><AffiliationInfo><Affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Neeraj</ForeName><Initials>N</Initials><Identifier Source="ORCID">0000-0001-6713-2129</Identifier><AffiliationInfo><Affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Jinhu</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McPhillips</LastName><ForeName>Scott E</ForeName><Initials>SE</Initials><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Davidson</LastName><ForeName>Catherine M</ForeName><Initials>CM</Initials><Identifier Source="ORCID">0000-0002-2523-4319</Identifier><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lyubimov</LastName><ForeName>Artem Y</ForeName><Initials>AY</Initials><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pence</LastName><ForeName>Natasha</ForeName><Initials>N</Initials><Identifier Source="ORCID">0000-0002-6096-4894</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schut</LastName><ForeName>Gerrit J</ForeName><Initials>GJ</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jones</LastName><ForeName>Anne K</ForeName><Initials>AK</Initials><Identifier Source="ORCID">0000-0003-4025-4809</Identifier><AffiliationInfo><Affiliation>School of Molecular Sciences , Arizona State University , Tempe , Arizona 85287 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Soltis</LastName><ForeName>S Michael</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Stanford Synchrotron Radiation Lightsource , SLAC National Accelerator Laboratory , Menlo Park , California 94025 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Adams</LastName><ForeName>Michael W W</ForeName><Initials>MWW</Initials><Identifier Source="ORCID">0000-0002-9796-5014</Identifier><AffiliationInfo><Affiliation>Department of Biochemistry and Molecular Biology , University of Georgia , Athens , Georgia 30602 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Raugei</LastName><ForeName>Simone</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-9118-8480</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>King</LastName><ForeName>Paul W</ForeName><Initials>PW</Initials><Identifier Source="ORCID">0000-0001-5039-654X</Identifier><AffiliationInfo><Affiliation>Biosciences Center , National Renewable Energy Laboratory , Golden , Colorado 80401 , United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peters</LastName><ForeName>John W</ForeName><Initials>JW</Initials><Identifier Source="ORCID">0000-0001-9117-9568</Identifier><AffiliationInfo><Affiliation>Institute of Biological Chemistry , Washington State University , Pullman , Washington 99164 , United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Chemistry and Biochemistry , Montana State University , Bozeman , Montana 59717 , United States.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Pacific Northwest National Laboratory , Richland , Washington 99352 , United States.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P41 GM103393</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Am Chem Soc</MedlineTA><NlmUniqueID>7503056</NlmUniqueID><ISSNLinking>0002-7863</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>12</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31816235</ArticleId><ArticleId IdType="doi">10.1021/jacs.9b08756</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Artz, Jacob H" sort="Artz, Jacob H" uniqKey="Artz J" first="Jacob H" last="Artz">Jacob H. Artz</name></noRegion><name sortKey="Adams, Michael W W" sort="Adams, Michael W W" uniqKey="Adams M" first="Michael W W" last="Adams">Michael W W. Adams</name><name sortKey="Artz, Jacob H" sort="Artz, Jacob H" uniqKey="Artz J" first="Jacob H" last="Artz">Jacob H. Artz</name><name sortKey="Cohen, Aina E" sort="Cohen, Aina E" uniqKey="Cohen A" first="Aina E" last="Cohen">Aina E. Cohen</name><name sortKey="Davidson, Catherine M" sort="Davidson, Catherine M" uniqKey="Davidson C" first="Catherine M" last="Davidson">Catherine M. Davidson</name><name sortKey="Ginovska, Bojana" sort="Ginovska, Bojana" uniqKey="Ginovska B" first="Bojana" last="Ginovska">Bojana Ginovska</name><name sortKey="Jones, Anne K" sort="Jones, Anne K" uniqKey="Jones A" first="Anne K" last="Jones">Anne K. Jones</name><name sortKey="Keable, Stephen M" sort="Keable, Stephen M" uniqKey="Keable S" first="Stephen M" last="Keable">Stephen M. Keable</name><name sortKey="King, Paul W" sort="King, Paul W" uniqKey="King P" first="Paul W" last="King">Paul W. King</name><name sortKey="Kumar, Neeraj" sort="Kumar, Neeraj" uniqKey="Kumar N" first="Neeraj" last="Kumar">Neeraj Kumar</name><name sortKey="Lyubimov, Artem Y" sort="Lyubimov, Artem Y" uniqKey="Lyubimov A" first="Artem Y" last="Lyubimov">Artem Y. Lyubimov</name><name sortKey="Mcphillips, Scott E" sort="Mcphillips, Scott E" uniqKey="Mcphillips S" first="Scott E" last="Mcphillips">Scott E. Mcphillips</name><name sortKey="Mulder, David W" sort="Mulder, David W" uniqKey="Mulder D" first="David W" last="Mulder">David W. Mulder</name><name sortKey="Pence, Natasha" sort="Pence, Natasha" uniqKey="Pence N" first="Natasha" last="Pence">Natasha Pence</name><name sortKey="Peters, John W" sort="Peters, John W" uniqKey="Peters J" first="John W" last="Peters">John W. Peters</name><name sortKey="Peters, John W" sort="Peters, John W" uniqKey="Peters J" first="John W" last="Peters">John W. Peters</name><name sortKey="Peters, John W" sort="Peters, John W" uniqKey="Peters J" first="John W" last="Peters">John W. Peters</name><name sortKey="Ratzloff, Michael W" sort="Ratzloff, Michael W" uniqKey="Ratzloff M" first="Michael W" last="Ratzloff">Michael W. Ratzloff</name><name sortKey="Raugei, Simone" sort="Raugei, Simone" uniqKey="Raugei S" first="Simone" last="Raugei">Simone Raugei</name><name sortKey="Raugei, Simone" sort="Raugei, Simone" uniqKey="Raugei S" first="Simone" last="Raugei">Simone Raugei</name><name sortKey="Schut, Gerrit J" sort="Schut, Gerrit J" uniqKey="Schut G" first="Gerrit J" last="Schut">Gerrit J. Schut</name><name sortKey="Soltis, S Michael" sort="Soltis, S Michael" uniqKey="Soltis S" first="S Michael" last="Soltis">S Michael Soltis</name><name sortKey="Song, Jinhu" sort="Song, Jinhu" uniqKey="Song J" first="Jinhu" last="Song">Jinhu Song</name><name sortKey="Williams, S Garrett" sort="Williams, S Garrett" uniqKey="Williams S" first="S Garrett" last="Williams">S Garrett Williams</name><name sortKey="Zadvornyy, Oleg A" sort="Zadvornyy, Oleg A" uniqKey="Zadvornyy O" first="Oleg A" last="Zadvornyy">Oleg A. Zadvornyy</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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